Co-dominant RAPD markers, observed as different-sized DNA segments amplified from the same locus, are detected only rarely. it is not possible to distinguish whether a DNA segment is amplified from a locus that is heterozygous (1 copy) or homozygous (2 copies). Nearly all RAPD markers are dominant, i.e.Selecting the right sequence for the primer is very important because different sequences will produce different band patterns and possibly allow for a more specific recognition of individual strains. Silver-stained polyacrylamide gel showing three distinct RAPD profiles generated by primer OPE15 for Haemophilus ducreyi isolates from Tanzania, Senegal, Thailand, Europe, and North America. RAPD is an inexpensive yet powerful typing method for many bacterial species. Therefore, if a mutation has occurred in the template DNA at the site that was previously complementary to the primer, a PCR product will not be produced, resulting in a different pattern of amplified DNA segments on the gel. For example, no fragment is produced if primers annealed too far apart or 3' ends of the primers are not facing each other. Unlike traditional PCR analysis, RAPD (pronounced "rapid") does not require any specific knowledge of the DNA sequence of the target organism: the identical 10-mer primers will or will not amplify a segment of DNA, depending on positions that are complementary to the primers' sequence. Markers are DNA fragments from PCR amplification of random segments of genomic DNA with single primer of arbitrary nucleotide sequence. Random Amplified Polymorphic DNA (RAPD) Introduction Random Amplified Polymorphic DNA (RAPD) Database of Single Nucleotide Polymorphisms (dbSNP).Database of Genomic Structural Variation (dbVar).Online Mendelian Inheritance in Man (OMIM).Database of Genotypes and Phenotypes (dbGaP).Structure (Molecular Modeling Database).Conserved Domain Search Service (CD Search).
BLAST (Basic Local Alignment Search Tool).Rapt: la séparation des races, a 1934 film directed by Dimitri Kirsanoff.Rapt (film), a 2009 French dramatic film directed by Lucas Belvaux.RATP Group ( Régie Autonome des Transports Parisiens), a public transport company based in Paris, France.RAPT1 or mTOR, the mammalian target of rapamycin protein.Retractable Amphibious Pontoon Technology, a retractable pontoon system for the float plane industry being developed by Tigerfish Aviation.Reverse Address and Port Translation, a variation of Network Address Translation in computing.Rehabilitation for Addicted Prisoners Trust (RAPt), a charity which helps people with drug and alcohol dependence move towards, achieve and maintain drug and crime-free lives.
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